Journal: American Journal of Human Genetics

Article Title: Loss of BRCC3 Deubiquitinating Enzyme Leads to Abnormal Angiogenesis and Is Associated with Syndromic Moyamoya

doi: 10.1016/j.ajhg.2011.04.017

Figure Lengend Snippet: Loss of brcc3 Function in the Endothelium Results in Defective Angiogenesis

Article Snippet: A polyclonal antibody specific for human BRCC3 was purchased from ProSci (reference number 4331F).

Techniques:

Journal: Oncology reports

Article Title: Radioresistant breast cancer cells exhibit increased resistance to chemotherapy and enhanced invasive properties due to cancer stem cells.

doi: 10.3892/or.2018.6714

Figure Lengend Snippet: Figure 2. RT‑R‑MDA‑MB‑231 cells demonstrate higher levels of CSCs marker CD44, but lower levels of CD24 compared with MDA‑MB‑231 cells. Classical CSCs markers CD44 and CD24 were detected in the low metastatic breast cancer cells MCF‑7 and RT‑R‑MCF‑7 (A) and highly metastatic breast cancer cells MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 (B) using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. **P<0.01 compared with control. CD, cluster of differentiation; CSCs, cancer stem cells; RT‑R, radioresistant.

Article Snippet: Isolation of CD24low/CD44high breast cancer stem cells was performed using a MagCollect CD24- CD44+ Breast Cancer Stem Cell Isolation kit (R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's protocol.

Techniques: Marker, Western Blot, Control

Journal: Oncology reports

Article Title: Radioresistant breast cancer cells exhibit increased resistance to chemotherapy and enhanced invasive properties due to cancer stem cells.

doi: 10.3892/or.2018.6714

Figure Lengend Snippet: Figure 3. RT‑R‑MDA‑MB‑231 cells increase the populations of CD24low/CD44high cells, and expression levels of other CSCs markers Notch‑4, Oct‑3/4 and ALDH1. (A) CD24low/CD44high cells were isolated from MDA‑MB‑231 and RT‑R‑MDA‑MB cells, and then the number of CD24low/CD44high breast cancer stem cells was quantified by flow cytometry. (B) MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 were labeled with anti‑CD24 and anti‑CD44 antibodies, and the percentages of CD24 or CD44‑expressed subpopulation in cells were determined by flow cytometry. (C) Other cancer stem cell markers, including Notch‑4, Oct3/4 and ALDH1 were detected in the MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 cells using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. *P<0.05 and **P<0.01 compared with MDA‑MB‑231 cells. ALDH1, aldehyde dehydrogenase 1; CD, cluster of differentiation; CSCs, cancer stem cells; Oct, octamer‑binding transcription factor; RT‑R, radioresistant.

Article Snippet: Isolation of CD24low/CD44high breast cancer stem cells was performed using a MagCollect CD24- CD44+ Breast Cancer Stem Cell Isolation kit (R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's protocol.

Techniques: Expressing, Isolation, Flow Cytometry, Labeling, Western Blot